This application discloses a data transmission method, a data transmission circuit, a display device and a storage medium, and relates to the field of display manufacturing. The method is used for a timing controller, the method including: sending preset link stability check data to a source driver after clock calibration; receiving feedback information sent by the source driver, wherein the feedback information is generated by the source driver when judging that the received link stability check data is correct; and sending target data to the source driver based on the feedback information.