Disclosed is a heart valve prosthesis (100), comprising a valved stent (110) and valve leaflets (190), wherein the valve leaflets (190) are accommodated in the valved stent (110), at least two valve leaflets(190) are provided and centrosymmetrically distributed along the circumferential direction of an inner surface of the valve leaflet stent (110), one ends of two adjacent valve leaflets (190) are combined together on the valve leaflet stent (110) to form a valve corner (192), the valved stent (110) is provided with a positioning member (1147), and the projections of a perpendicular line of one of the valve corners (192) to the axis of the valved stent (110) and a perpendicular line of the positioning member (1147) to the axis of the valved stent (110) are coincident on a plane perpendicular to the axis of the valved stent (110). The heart valve prosthesis (100) can considerably reduce regurgitation at the center.