The present application discloses a method, device and system for data transmission, and a display device, and belongs to the field of manufacturing of a liquid crystal panel. The method is applied to a controller. The controller is connected to a plurality of driver chips connected in parallel via a first signal line, and the plurality of driver chips is sequenced according to a preset response feedback sequence. The method comprises: generating a data request instruction, wherein the data request instruction includes the identity of a start driver chip, and the start driver chip is a driver chip in the plurality of driver chips; sending, via the first signal line, a data request instruction; receiving, via the first signal line, the data response instructions sequentially sent by the plurality of driver chips from the start driver chip according to the response feedback sequence, wherein each of the data response instructions comprises an identity of a corresponding driver chip and data of the corresponding driver chip. The present application solves the problem that the first signal line has a single function and a relatively low utilization rate in the related art, enriches the function of the first signal line, improves the utilization rate, and is used for a display device.