The invention provides a lateral flow detection device for detecting a coronavirus by immunoassay, wherein the detection device comprises two lateral flow test strips, a test strip 1 is used to test an antibody to a N full-length protein and/or a S full-length protein, while a test strip 2 is used to test an antibody to a S-RBD site protein, and a combination of the two test strips is used to test IgG and IgM antibodies to novel coronavirus, which further improves a detection rate of serological antibodies and effectively reduces a possibility of missing detection and wrong detection, thereby avoiding any missing detection. Simultaneously, the device could be further used to identify an effective antibody produced by an innate immunity and/or after an patient's recovery from disease, so the two test strips are significantly complementary to each other. The detection device of the present invention has the advantages that it detects reliably and efficiently in a simple manner; it can prevent missing detection as possible, it operates conveniently without need of professional instruments and personnel, and easily popularized for clinical promotion and application. Further, the present invention provided two antigen combinations for preparing detection devices for rapid detection of novel coronavirus and an application thereof.