发明公开
- 专利标题： CELL-CONTAINING VESSEL AND METHOD FOR PRODUCING NEURAL CELL-CONTAINING SPHEROID
-
申请号： EP21175414.8申请日： 2021-05-21
-
公开(公告)号： EP3916083A1公开(公告)日： 2021-12-01
- 发明人: IJICHI, Rie , ARATANI, Tomoyuki , NAKAYAMA, Tomoaki , KOSHIZUKA, Shinnosuke
- 申请人： Ricoh Company, Ltd.
- 申请人地址： JP Tokyo 143-8555 3-6, Nakamagome, 1-chome Ohta-ku
- 代理机构： Fairbairn, Angus Chisholm
- 优先权： JP2021062866 20210401
- 主分类号： C12N5/071
- IPC分类号： C12N5/071 ; C12N5/0793 ; C12N5/079 ; G01N33/50
摘要：
The invention provides a cell-containing vessel comprising a plurality of neural cell-containing spheroids for which the variation calculated using the calculation method described below is less than 20%, wherein the neural cell-containing spheroids contain a plurality of types of cells including neural cells,
(Calculation Method)
Calcium transient assays are conducted for each of a plurality of neural cell-containing spheroids, a number of spontaneous oscillations in a 10-minute period is measured, an average and standard deviation for the number of spontaneous oscillations are calculated, and a variation is calculated using formula (1) below: Variation % = standard deviation for number of spontaneous oscillations / average number of spontaneous oscillations × 100
(Calculation Method)
Calcium transient assays are conducted for each of a plurality of neural cell-containing spheroids, a number of spontaneous oscillations in a 10-minute period is measured, an average and standard deviation for the number of spontaneous oscillations are calculated, and a variation is calculated using formula (1) below: Variation % = standard deviation for number of spontaneous oscillations / average number of spontaneous oscillations × 100
信息查询
