Provided is a novel porous cellulose medium that can efficiently separate a large target molecule in a calibration standard having a large pore diameter and a large pore volume and a weight average molecular weight from approximately 10 5.0 to 10 5.5 . A porous cellulose medium including a porous cellulose particle having a particle size from 1 to 600 µm, wherein, in sieving the porous cellulose medium for classification and using a fraction corresponding to aperture openings between 53 µm and 106 µm as a support for size exclusion chromatography, a polyethylene oxide standard is run through size exclusion chromatography with pure water as a mobile phase, and a weight average molecular weight Mw and a gel partition coefficient K av of the polyethylene oxide standard satisfy Relationships (A) and (B) above:
in a case where 4 . 80 ≤ logMw ≤ 5 . 50 , K av > − 0.445 × logMw + 2.55
in a case where 5 . 75 ≤ logMw , 0 ≤ K av 0.19