Provided are a microfluidic chip and a manufacturing method thereof, and a microfluidic device, and relates to the field of microfluidic technology. The microfluidic chip comprises a first substrate structure comprising a plurality of pin areas comprising a first and a second pin area, a detection area, and a grounding trace. The detection area comprises a plurality of first scan lines extending along a first direction, each of which being connected to the first pin area through a corresponding first scan trace; a plurality of first data lines extending along a second direction different from the first direction, each of which being connected to the second pin area through a corresponding first data trace; a plurality of detection units, each of which comprising a first switching transistor connected to a corresponding first scan line and data line, a driving electrode connected to the first switching transistor, and a first hydrophobic layer above the driving electrode. The grounding trace is connected to at least one detection unit and one of the plurality of pin areas.