Provided are compositions comprising a recombinant phenylalanine ammonia-lyase (PAL) derived from the bacterial species Anabaena variabilis, Nostoc punctiforme, Streptomyces maritimus, Streptomyces verticillatus, Soragium cellulosum, Nostoc tobacum, or Pseudomonas putida, or a biologically active fragment, mutant, variant or analog thereof, and a pharmaceutically acceptable carrier. Further provided are such compositions of recombinant bacterial PAL and biologically active fragments, mutants, variants or analogs thereof for use in treating phenylketonuria hyperphenylalaninemia.