A method with biometric information spoof detection includes extracting an embedding vector from an intermediate layer of a neural network configured to detect whether biometric information of a user is spoofed from an image including the biometric information; detecting first information regarding whether the biometric information is spoofed, based on the embedding vector; and detecting second information regarding whether the biometric information is spoofed based on whether the first information is detected, using an output vector output from an output layer of the neural network.