A color conversion substrate includes: a bank having a first open region and a second open region; a color conversion layer in the first opening region, the color conversion layer including color conversion particles; a first partition wall partitioning the second opening region into a central region and a peripheral region surrounding the central region, the first partition wall having a polygonal shape with n vertices in a plan view where n is a natural number greater than or equal to 3; and n second partition walls extending from the n vertices of the first partition wall to an edge of the second opening region, respectively, to partition the peripheral region into n sub-regions.