A base substrate (111) of a display substrate (110) includes a display region (1111); a plurality of pixel units (112) in the display region (1111), wherein each pixel unit (112) includes a pixel circuit (1121) including a first pixel circuit (1121a) and a second pixel circuit (1121b) adjacent to each other in a first direction; an initialization signal line (113) extending in the first direction and supplying an initialization signal to the first pixel circuit (1121a) and the second pixel circuit (1121b); a first control signal line (114A) extending in the first direction and supplying gate signals to the first pixel circuit (1121a) and the second pixel circuit (1121b); and a light-emitting control signal line (115) extending in the first direction and supplying light-emitting control signals to the first pixel circuit (1121a) and the second pixel circuit (1121b); the initialization signal line (113) is on a side of the light-emitting control signal line (115) away from the first control signal line (114A), extends along the first direction and includes segments arranged at intervals.