A method of culturing human induced pluripotent stem cells includes a step of inoculating human induced pluripotent stem cells in a culture medium at an inoculation density of 1.0 × 10 4 to 1.0 × 10 6 cells/cm 2 in a culture vessel and subjecting the human induced pluripotent stem cells to two-dimensional culturing. A method of producing cerebral organoids includes a step 1 of culturing a culture of the human induced pluripotent stem cells obtained by the method of culturing human induced pluripotent stem cells in a culture medium containing a BMP inhibitor and a transforming growth factor β (TGFβ) inhibitor to form cell aggregates, a step 2 of culturing the cell aggregates in a culture medium containing a Wnt signal transduction pathway potentiator and an extracellular matrix, and a step 3 of subjecting the culturing obtained in the step 2 to spinner culturing.