The present application provides an application window adjustment method and apparatus, a device, and a medium. The method includes: displaying a target application window in a virtual space in response to a selection instruction for a target application; when a trigger operation for a target button on a controller is detected, determining a display position of a cursor corresponding to the controller; and if the display position of the cursor is located on the target application window, adjusting the target application window based on a movement trajectory of the cursor, where the display position of the cursor varies with a position of the controller. According to the present application, the purpose of adjusting a 2D application window in a virtual space is achieved, so that the need of a user to adjust the 2D application window is met, and the user experience is improved.