制作微阵列的过程。 该方法包括在具有至少一个基准的基底上沉积一群微珠的步骤。 群体由至少两个亚群组成，优选多个亚群，每个亚群包含能够与至少一种目标分析物特异性结合的已知活性剂。 所述亚群是以彼此不连续的时间顺序地沉积的。 该方法还包括在每个亚群沉积后制作基底的图像的步骤。 然后使用基准作为参考来比较图像，从而基于每个图像之间的差异来确定每个微珠的位置并识别亚群及其已知的活性剂。 还在用于使用微阵列的系统中公开。

A method for manufacturing a microarray. Said method comprising the steps of placing a population of microbeads on a substrate having at least one reference. The population at least 2 subpopulations, preferably of a number of subpopulations, each comprising a known active substance capable of specifically binding at least one target analyte. The subpopulation are sequentially disposed on each other separate periods. The method also includes the step of creating an image of the substrate after placing each subpopulation. Then, by comparing the image with the reference as a reference, it determines the position of the microbeads each identified subpopulations and known active substances that based on the differences between each image. System for the use of the microarray are also disclosed.