The present invention is Kringle domain structure comprising the step of inducing artificial mutations at amino acid residues except the important conserved amino acid residues in maintaining the Kringle domain structure skeleton, and by specifically binding to the target molecule provides a protein scaffold mutant kringle domains infrastructure that modulate the biological activity of various target molecules derived from the protein scaffold library. In addition, the present invention provides a method for constructing a homo / hetero-oligomeric to multi-target and multi-specifically bound by a tandem (tandem) assembly of mono mark ring Lumpur domain mutant with a linker. Further, the present invention provides a targeted binding loops of Kringle domain variants, by grafting the non-binding loops of other kringle domain variants having the same or different target binding specificity, multispecific monomers and multiple bonds monomer to provide a method for producing. Further, the present invention comprises administering protein scaffold variants of the Kringle domain infrastructure that specifically binds to a target molecule, DNA encoding the protein scaffold variant, or an effective amount of related molecules animal, preferably a human include, diseases or disorders, in particular preventing cancer and other immune related diseases, detection, diagnosis, provides methods and compositions for treating or alleviating.