The present disclosure, for example, for vaccine production, relates to a method of producing the enterovirus A, the method comprises culturing the cells in a fixed bed bioreactor. Further in this specification, enterovirus A manufactured by the methods disclosed herein, as well as compositions related thereto, immunogenic compositions, and vaccines are disclosed. Methods of making the containing enterovirus A virus: (a) the adherent cells, the method comprising culturing in a fixed layer comprising a macro carrier, the cells are to be cultured in the first cell culture medium ( under conditions b) the enterovirus a virus infects a cell, the possible enteroviruses a virus inoculated into cell, (c) under conditions which infected cells produce enterovirus a virus-infected cells a second cell culture in the medium, culturing the fixed layer, and (d) said recovering enteroviruses a virus produced by the cell.
.FIELD 7