Advance manufacturing a plurality of dimer probes, by increasing the kind, than ever to shorten the reaction time of the self-assembly substance formed, and, allows to increase the area of ​​the probe that can be used to detect the target gene to provide a new probe self-assembly manufacturing method was. Each pair of oligonucleotides, 3 divided into three regions of 'side region, central region, and the 5' side region, a central region of each oligonucleotide is complementary to the nucleotide sequence to each other, the 3 'side region, and 5' by forming a plurality of systems including a pair of dimer-forming probes with non-complementary base sequence to each other side region, hybridizing a plurality of pairs of dimer forming probes in a plurality of systems, oligonucleotides self-assemble and were so as to form a self-assembly of double stranded.