The present invention relates to a method for producing nerve cells by inducing differentiation of embryonic stem cells, a method of inducing differentiation of embryonic stem cells into neural cells, is differentiation-inducing medium used in the production process or method for inducing differentiation, or embryonic stem cells, and to provide a method for increasing the purity of the nerve cells. According to the present invention, by inducing differentiation by substance or a salt thereof with embryonic stem cells vitamin B12 or salt and heparin or heparin-like action of those nerve cells that can be applied in the treatment of such neurodegenerative diseases the simple, there is provided selective or method of inexpensively manufactured.