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KR102426954B1 대류 PCR 증폭 탐지 시스템 및 대류 PCR 증폭 탐지 방법 有权

  • 专利标题： 대류 PCR 증폭 탐지 시스템 및 대류 PCR 증폭 탐지 방법
  • 专利标题（英）： SYSTEM FOR DETECTING CONVECTIVE PCR AMPLIFICATION AND METHOD FOR DETECTING CONVECTIVE PCR AMPLIFICATION
  • 申请号： KR1020197023956
    申请日： 2018-01-16
  • 公开(公告)号： KR102426954B1
    公开(公告)日： 2022-08-01
  • 优先权： CN2017100547981 2017-01-24
  • 国际申请： PCT/CN2018/072833 2018-01-16
  • 国际公布： WO2018137513 2018-08-02
  • 主分类号： B01L3/00
  • IPC分类号： B01L3/00 ;  B01L7/00 ;  C12Q1/686
대류 PCR 증폭 탐지 시스템 및 대류 PCR 증폭 탐지 방법
摘要：
본출원은대류 PCR 증폭탐지시스템및 방법을제공한다. 상기시스템은저장구조물, 대류 PCR 튜브, FTA 막및 폐기액체수용구조물을포함하고, 상기대류 PCR 튜브의제 1 단부는상기저장구조물의저장공동과연결되고, 대류 PCR 튜브의제 2 단부는폐기액체수용구조물의폐기액체공동과연결되고, 상기 FTA 막은대류 PCR 튜브의제 1 단부로부터제 2 단부로유동하는용액을여과하기위해대류 PCR 튜브내에배치되며, 상기용액내의핵산을상기 FTA 막의표면상에흡착시킬수 있는, 미세유체칩; 저장공동내의용액을대류 PCR 튜브로진입하게하고 FTA 막에의해이를여과한후에폐기액체공동에용액이들어가도록하기위한, 유동제어모듈; 대류 PCR 튜브에서반응물질을가열하기위한, 가열모듈; 및대류 PCR 튜브내의반응물질상의형광탐지에수행하기위한, 광학탐지모듈을포함한다. 본출원은핵산분석의효율을향상시킬수 있다.
摘要（英）：

Disclosed are a system and a method for detecting convective PCR amplification. The system comprises: a microfluidic chip, comprising a storage structure, a convective PCR tube, an FTA film and a waste liquid receiving structure, wherein a first end of the convective PCR tube is in communication with a storage cavity of the storage structure, a second end of the convective PCR tube is in communication with a waste liquid cavity of the waste liquid receiving structure, and the FTA film is arranged in the convective PCR tube so as to filter a solution flowing from the first end of the convective PCR tube to the second end of same, and is capable of adsorbing nucleic acids in the solution onto the surface of the FTA film; a flow control module, used for making a solution in the storage cavity flow into the convective PCR tube and for making same be filtered via the FTA film, and then enter the waste liquid cavity; a heating module, used for heating materials in the convective PCR tube; and an optical detection module, used for the fluorescence detection of materials in the convective PCR tube.

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