The present disclosure relates to systems and methods for determining an integrity of a sample chamber. In certain embodiments, formation fluid is collected from a subterranean formation within a sample chamber disposed in a downhole tool, the downhole tool is withdrawn from a wellbore, an estimated surface pressure of the collected formation fluid is determined, the estimated surface pressure of the collected formation fluid is compared with an actual surface pressure of the sample chamber, and the integrity of the sample chamber is determined based on the comparison of the estimated surface pressure and the actual surface pressure.