The present application discloses a touch display substrate including an array of a plurality of pixels. Each pixel includes a first region and a second region in plan view of the touch display substrate. Each pixel includes a first electrode layer on a base substrate comprising a plurality of first electrode blocks in the first region, each of which corresponding to a subpixel; and a second electrode block in the second region; a first light emitting layer in the first region on a side of the plurality of first electrode blocks distal to the base substrate; a second light emitting layer in the second region on a side of the second electrode block distal to the base substrate; a second electrode layer in the first region on a side of the first light emitting layer distal to the plurality of first electrode blocks; and a touch electrode layer in the second region on a side of the second light emitting layer distal to the second electrode block; the touch electrode layer and the second electrode layer spaced apart and electrically insulated from each other.