Technologies are generally described for spectroscopic determination of one or more optical properties of a gemstone. An imaging device may include one or more light sources configured to illuminate one or more portions of the gemstone, and one or more photo detectors configured to detect reflected light from the portions of the gemstone in response to the illumination. An analysis module may be communicatively coupled to the imaging device, and configured to analyze the reflected light to determine the optical properties of the portions of the gemstone. The optical properties may include at least one of a clarity, color, fluorescence, birefringence, dichroism, and brilliance of the portions of the gemstone. In some examples, an optical fingerprint of the gemstone may be created based on one or more determined optical characteristics of the portions of the gemstone, where the optical fingerprint may uniquely identify the gemstone.