Invention Grant
- Patent Title: Planar patch clamp device, electrodes for said device and cell ion channel current measurement method
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Application No.: US14366708Application Date: 2012-12-05
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Publication No.: US10114005B2Publication Date: 2018-10-30
- Inventor: Tsuneo Urisu , Zhi-hong Wang , Hidetaka Uno , Senthil Kumar Obuliraj , Yasutaka Nagaoka
- Applicant: Japan Science and Technology Agency
- Applicant Address: JP Kawaguchi-Shi
- Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
- Current Assignee: JAPAN SCIENCE AND TECHNOLOGY AGENCY
- Current Assignee Address: JP Kawaguchi-Shi
- Priority: JP2011-278445 20111220; JP2012-181786 20120820
- International Application: PCT/JP2012/081556 WO 20121205
- International Announcement: WO2013/094418 WO 20130627
- Main IPC: G01N33/487
- IPC: G01N33/487 ; G01N27/416

Abstract:
An object of the present invention is that variations in an applied membrane potential in the planar patch clamp device are suppressed to reduce a noise current, thereby enabling accurate measurement of an ion channel current.Disclosed is a planar patch clamp device including: an electrically insulative substrate provided with one or more fine through holes; a liquid reservoir that holds a conductive liquid provided on both surface sides of the through hole; and energizable electrode sections provided in the liquid reservoir; these electrode sections including: (a) an electrode vessel, at least part of which is made of an inorganic porous material, (b) an electrode in which a chloride NmCl layer is formed on the surface of a noble metal Nm, and (c) an electrode solution containing NmCl and an alkali metal chloride being dissolved therein at a saturated concentration.
Public/Granted literature
- US20140339102A1 PLANAR PATCH CLAMP DEVICE, ELECTRODES FOR SAID DEVICE AND CELL ION CHANNEL CURRENT MEASUREMENT METHOD Public/Granted day:2014-11-20
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