Methods and compositions for nuclease-mediated targeted integration of transgenes

Invention Grant

US10174331B2 Methods and compositions for nuclease-mediated targeted integration of transgenes 有权

Patent Title: Methods and compositions for nuclease-mediated targeted integration of transgenes
Application No.: US13889162

Application Date: 2013-05-07
Publication No.: US10174331B2

Publication Date: 2019-01-08
Inventor: Gregory J. Cost , Fyodor Urnov , W. Michael Ainley , Joseph F. Petolino , Jayakumar Pon Samuel , Steven R. Webb , Lakshmi Sastry-Dent
Applicant: Sangamo BioSciences, Inc. , Dow AgroSciences LLC
Applicant Address: US CA Richmond US IN Indianapolis
Assignee: Sangamo Therapeutics, Inc.,Dow AgroSciences LLC
Current Assignee: Sangamo Therapeutics, Inc.,Dow AgroSciences LLC
Current Assignee Address: US CA Richmond US IN Indianapolis
Agency: Pasternak Patent Law
Main IPC: C12N15/82
IPC: C12N15/82 ; C12N15/87 ; C12N15/85 ; C12N15/10

Abstract:

Disclosed herein are methods and compositions for homology-independent targeted insertion of donor molecules into the genome of a cell.

Public/Granted literature

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Espacenet

IPC分类:

C	化学；冶金
C12	生物化学；啤酒；烈性酒；果汁酒；醋；微生物学；酶学；突变或遗传工程
C12N	微生物或酶；其组合物；繁殖、保藏或维持微生物；变异或遗传工程；培养基（微生物学的试验介质入C12Q1/00）。
C12N15/00	突变或遗传工程；遗传工程涉及的DNA或RNA，载体（如质粒）或其分离、制备或纯化；所使用的宿主（突变体或遗传工程制备的微生物本身入C12N1/00、C12N5/00、C12N7/00；新的植物入A01H；用组织培养技术再生植物入A01H4/00；新的动物入A01K67/00；含有插入活体细胞的遗传物质以治疗遗传疾病的药剂的应用，基因疗法入A61K48/00，一般肽入C07K）
C12N15/09	.DNA重组技术
C12N15/63	..使用载体引入外来遗传物质；载体；其宿主的使用；表达的调节
C12N15/79	...专门适用于真核细胞宿主的载体或表达系统
C12N15/82	....用于植物细胞