The present disclosure discloses an organic light emitting diode substrate, comprising: a base substrate and a device layer formed in a middle part thereon, a region on the base substrate other than the device layer being divided into an inner ring region that surrounds the device layer and an outer ring region outside the inner ring region; a first adhesive layer adhered on the device layer and the inner ring region and covering the device layer and the inner ring region, the first adhesive layer being used to block moisture and oxygen for the device layer; a second adhesive frame adhered on the outer ring region and the first adhesive layer and covering a portion of the first adhesive layer over the inner ring region, the second adhesive frame having a higher adhesive strength than the first adhesive layer.