A pedestal level for an image sensor can be dynamically adjusted based on one or more parameters. The parameters include one or more operating conditions associated with the image sensor, pre-determined image sensor characterization data, the number of unused digital codes, and/or the number of clipped pixel signals. The operating conditions can include the temperature of the image sensor, the gain of at least one amplifier included in processing circuitry operably connected to at least one pixel, and/or the length of the integration period for at least one pixel in the image sensor. Based on the one or more of the parameters, the pedestal level is adjusted to reduce a number of unused digital codes in a distribution of dark current. Additionally or alternatively, the variance of the pixel signals can be reduced to permit the use of a lower pedestal level.