A micro heating device according to an embodiment of the inventive concept comprises a support part having at least one or more heating part, an oil chamber positioned over the support part and filled with oil therein, a specimen chamber having a reaction space into which a specimen is loaded and which is provided so as to be dipped into the oil, and a drive part configured to move the specimen chamber in the oil. The specimen chamber includes a temperature sensor for measuring a temperature of the specimen chamber.