A method for tracking a biological cell in an image sequence with at least a first image and a second image includes the steps of: identifying a plurality of biological cells in the first image and a plurality of biological cells in the second image; determining matching probability of a biological cell in the second image with a biological cell in the first image, based on relative positional relationships between the plurality of biological cells in the first image and relative positional relationships between the plurality of biological cells in the second image; and determining, based on the determined matching probability, a matching result relating to whether the biological cell in the second image matches with the biological cell in the first image. A match indicates that the biological cell in the second image corresponds with or originates from the corresponding biological cell in the first image.