Technologies for cryptographic protection of I/O audio data include a computing device with a cryptographic engine and an audio controller. A trusted software component may request an untrusted audio driver to establish an audio session with the audio controller that is associated with an audio codec. The trusted software component may verify that a stream identifier associated with the audio session received from the audio driver matches a stream identifier received from the codec. The trusted software may program the cryptographic engine with a DMA channel identifier associated with the codec, and the audio controller may assert the channel identifier in each DMA transaction associated with the audio session. The cryptographic engine cryptographically protects audio data associated with the audio session. The audio controller may lock the controller topology after establishing the audio session, to prevent re-routing of audio during a trusted audio session. Other embodiments are described and claimed.