A subject observation system includes a light source and an image obtaining unit. The light source emits observation light including a spectral component of a wavelength and applies the light to a subject. The image obtaining unit images reflected light from an irradiation region of the subject to which the light has been applied and obtains at least two observation images in different wavelength regions based on image signals corresponding to a blue region, a green region and a red region. The light includes components of light emission spectra in the regions. The light emission spectrum in the blue region is smaller in a wavelength region in which absorption intensity for a specific observation target in the subject is relatively low than in other regions.