Invention Grant
- Patent Title: Synthetic long read DNA sequencing
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Application No.: US15027069Application Date: 2014-09-24
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Publication No.: US10266904B2Publication Date: 2019-04-23
- Inventor: Jeremy Scott Edwards
- Applicant: STC.UNM
- Applicant Address: US NM Albuquerque
- Assignee: STC.UNM
- Current Assignee: STC.UNM
- Current Assignee Address: US NM Albuquerque
- Agency: Mueting, Raasch & Gebhardt, P.A.
- International Application: PCT/US2014/057260 WO 20140924
- International Announcement: WO2015/053943 WO 20150416
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12Q1/689 ; C12Q1/6853

Abstract:
The disclosure describes a method for sequencing long portions of DNA sequence by assembling a plurality of shorter polynucleotide reads. Generally, The method includes annealing a plurality of primers to a denatured DNA molecule, appending a barcode polynucleotide to the 5′ end of the primer, subjecting the DNA molecules to a plurality of cycles of (1) pooling, (2) dividing, and (3) appending a barcode polynucleotide to the 5′ end of the primer, sequencing the barcode polynucleotides and the genomic DNA, and assembling the short read polynucleotide sequences having identical barcode polynucleotides.
Public/Granted literature
- US20160244811A1 SYNTHETIC LONG READ DNA SEQUENCING Public/Granted day:2016-08-25
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