The subject matter described herein includes methods, systems, and computer readable media for automated generation of test files and for related testing. According to one method, the method includes receiving a configuration file, where the configuration file indicates a test case or a test plan to be generated, wherein the configuration file includes a feature identifier or a bug identifier and information about a system under test (SUT). The method also includes generating at least one test file based on the configuration file, wherein the at least one test file is for testing a feature or a bug in the SUT associated with the feature identifier or the bug identifier in the configuration file. The method further includes generating at least one directory based on the configuration file and storing the at least one test file in the at least one directory. The method also includes testing the feature or bug in the SUT using the at least one test file.