The present disclosure provides a display substrate and a method of fabricating the same, a display panel and a pressure welding device. The display substrate includes a flexible substrate having a first surface and a second surface opposite to each other, and the first surface includes a first area and a second area. The method includes forming thin film transistors and light-emitting elements in the first area, forming a lead for circuit-bonding in the second area, forming a curable material layer on the second surface, and performing a curing process on a part of the curable material layer corresponding to the second area to form a cured layer. The technical solutions of the present disclosure improves the stability of pressure welding during a pressure welding process of circuit component, and lowers the possibility of occurrence of wire defect.