The present disclosure relates to the field of display technology, and discloses a substrate and a method for fabricating the same, and a display device. The substrate includes a plurality of players of conductive lines arranged on a base, and adjacent two layers of conductive lines are arranged with a signal shielding layer there between to eliminate signal crosstalk between the adjacent two layers of conductive lines. And in the direction of arrangement of the same layer of conductive lines, projections of regions of distribution of adjacent two layers of conductive lines on the base are at least partially overlapped with each other.