An air-shielded fuel injection assembly for use in a combustion chamber of a turbine assembly. The air-shielded fuel injection assembly generally includes a fuel manifold including a plurality of fuel injection ports and an air manifold including a plurality of air injection ports. Each of the plurality of fuel injection ports is configured to introduce a fuel column into an annular cavity of a mixer assembly. Each of the plurality of air injection ports is configured to introduce an air curtain about an associated fuel injection column to minimize recirculation upstream of the fuel injection column and increase penetration of the fuel injection column into the cavity. Also disclosed are a mixer assembly and a turbine assembly including the air-shielded fuel injection assembly.