Provided are a light diffusion film having a single-layered light diffusion layer, in which the uniformity of the intensity of diffused light in the light diffusion angle region can be increased, or the light diffusion angle region can be expanded effectively, by regulating the combination of the angles of inclination of pillar-shaped objects in plural columnar structure regions, and a method for manufacturing a light diffusion film. Disclosed is a light diffusion film having a single-layered light diffusion layer, the light diffusion film having a first columnar structure region and a second columnar structure region, in which plural pillar-shaped objects having a relatively high refractive index are arranged to stand close together in a region having a relatively low refractive index, sequentially from the lower part of the film along the film plane.