A transfer plate and an orientation film generation system are provided. The transfer plate includes a bottom plate and a plurality of relief plates, wherein, the bottom plate is disposed on a printing cylinder and is distributed with a plurality of engagement holes, each of the engagement holes has a plurality of stopper locations configured to provide different engagement depths; and the plurality of relief plates and the plurality of engagement holes are disposed in one-to-one correspondence, and each of the relief plates is provided with an engagement pin cooperated with the stopper locations of a corresponding engagement hole, wherein the relief plate is made of permeable materials. The orientation film generation system includes the above transfer plate.