Embodiments of the present invention provides an array substrate. The array substrate includes a display region and a packaging region. The packaging region includes a plurality of functional layers. And the packaging region further includes: a plurality of through holes running through at least one of the plurality of functional layers and configured to allow a packaging adhesive to enter therein; and a groove formed above at least some of the through holes, wherein, projection areas of the at least some of the through holes onto a base substrate of the array substrate are located within a projection area of the groove onto the base substrate. Embodiments of the present invention further provides a display panel and a display apparatus including the abovementioned array substrate, and a method of manufacturing the abovementioned array substrate.