The present application discloses a method of fabricating a display substrate. The method includes forming a sacrificial layer including a plurality of sacrificial blocks on a base substrate; forming a pixel definition material layer on the base substrate subsequent to forming the sacrificial layer; and removing the sacrificial layer thereby forming a pixel definition layer comprising a plurality of pixel definition blocks. Each of the plurality of sacrificial blocks is formed to have a first side surface distal to the base substrate, a second side surface facing the first side surface and proximal to the base substrate, and a third side surface connecting the first side surface and the second side surface. An average tangential inclination of the third side surface with respect to the second side surface is an acute angle.