A dust cup assembly (100) and a handheld cleaner (1000) having the same are provided. The dust cup assembly (100) includes: a cup casing (1); a device housing (2) configured as a tube shape and disposed in the cup casing (1), wherein an outer end face of the device housing (2) at an axial side thereof abuts against or extends beyond a partial inner surface of the cup casing (1), and a dust removal chamber (A1) is defined between an inner surface of the cup casing (1) and an outer peripheral surface of the device housing (2) and surrounds the device housing (2) along a circumferential direction of the device housing (2); and a negative pressure device (3) located within the device housing (2) and enabling dusty air to enter the dust removal chamber (A1) for dust and air separation.