Methods, systems, and computer-readable media for virtual desktop access using device-native user interfaces are disclosed. A virtual desktop instance is implemented on behalf of a user. One or more applications are installed on the virtual desktop instance and executed using a virtualized computing resource instance. Data associated with the virtual desktop instance is sent to a first user device that implements a first device platform. A first graphical user interface (GUI) for the virtual desktop interface is generated using the data and displayed on a first display of the first device. The data is sent to a second user device that implements a second device platform differing from the first device platform. A second GUI for the virtual desktop interface is generated using the data and displayed on a second display of the second device. The second GUI differs at least in part from the first GUI.