A printing apparatus includes: a conveyor that conveys a medium including a base layer and a separation layer and including a first region, a second region, and a third region; a printer; and a controller. The controller is configured to control the printer to print a first mark on a portion of the medium in one of the first region and a first-region-side portion of the second region and to print a second mark on a portion of the medium in one of the third region and a third-region-side portion of the second region such that the first mark and the second mark are arranged in a line in the first direction.