Web Bot detection methods and systems are provided that receive a request, in connection with a network session. The methods and systems determine whether the request is associated with potential Bot activity, and based thereon assign a Bot confidence designation. The Bot confidence designation indicates a likelihood that the request represents an agent-based request. The methods and systems analyze a session trait of the network session relative to predetermined session traits indicative of human-based requests, and assign a human confidence designation based on the analysis. The human confidence designation indicates a likelihood that the request represents a human-based request. The request is then classified to represent an agent-based request or human-based request based on the Bot and human confidence designations.