The present invention provides a touch substrate, a manufacturing method thereof, and a display device. In the touch substrate of the present invention, first leads of a first-layer structure are connected with first patterns in a second-layer structure via first via holes, second leads of the first-layer structure are connected with second patterns in the second-layer structure via second via holes, and the first patterns and the second patterns produce mutual capacitance. Each first pattern comprises a plurality of branches radiating from the center to the circumference, each second pattern surrounds one first pattern in a mutually separated manner, and when the touch substrate is used for flexible display, the second patterns can cover the whole bent surface, so that when a user touches any place of the touch substrate, the touch substrate can quickly respond and blind spots cannot be formed.