An impedance tube and sample tube holder that includes a hollow tube, a first tube, a second tube, and a spool. The hollow tube has two halves which are detachable and can hold spacers and a sample. The first tube includes a first tube speaker end and a first tube spool end. The first tube has a speaker disposed at the first tube speaker end and microphones. The second tube includes an anechoic terminator tube end and a second tube spool tube. The second tube has microphones and an anechoic terminator at the anechoic terminator tube end. The spool holds the hollow tube with the spacers and the sample. The spool is attachable to the first tube spool end and the second tube spool end such that the sample is perpendicularly orientated to an incoming sound wave produced by the speaker.