A display driving circuit, a calibration module, and an associated calibration method are provided. The display driving circuit includes an internal clock circuit and the calibration module. The internal clock circuit generates an internal clock signal. The calibration module includes a counting circuit and a trimming circuit. The counting circuit counts pulses of a reference clock signal to generate a detected reference-clock count and counts pulses of the internal clock signal to generate a detected internal-clock count. The trimming circuit generates a calibration signal to adjust frequency of the internal clock signal when a predefined condition is satisfied. The predefined condition is related to comparison between a first preset count and one of the detected reference-clock count and the detected internal-clock count.