A method and an apparatus for transmitting uplink demodulation reference signals (DMRSs) is provided. The method includes a user equipment (UE) determines an uplink DMRS format for demodulating a physical uplink shared channel (PUSCH) according to frequency-domain resources occupied by the PUSCH, in which the uplink DMRS format includes a comb occupied by a PUSCH DMRS sequence. The comb is subcarriers having specified intervals occupied by demodulation reference signals, and the specified intervals between the occupied subcarriers are same. For the DMRSs using the comb format, subcarriers with specific intervals are used for channel estimation, and values of the channel estimation are used for data demodulation. The UE transmits uplink information and the demodulation reference signals on physical resources using the uplink DMRS format. The present disclosure can improve the multiplexing ratio of uplink physical resources in a multi-UE scenario.