Provided are a phosphor-containing capable of suppressing deterioration of phosphors and can be manufactured with high efficiency and a backlight unit. Specifically, provided is a phosphor-containing film, including a first substrate film; and a phosphor-containing layer at which a plurality of regions containing phosphors, which, if exposed to oxygen, deteriorate by reacting with the oxygen, are discretely disposed on the first substrate film, and at which a resin layer having an impermeability to oxygen is disposed between the discretely disposed regions containing phosphors, in which a width S of the resin layer between the regions containing phosphors is 0.01≤S