A display substrate, a manufacturing method thereof, and a display panel are provided. The display substrate includes a base substrate, a display region on the base substrate and a wiring region surrounding the display region. The wiring region includes at least one signal line and at least one dummy line arranged parallel to the signal line. A distance between a surface of the dummy line away from the base substrate and the base substrate is greater than a distance between a surface of the signal line away from the base substrate and the base substrate. The dummy line is arranged along the signal line, and the distance between the surface of the dummy line and the base substrate is larger than the distance between the surface of the signal line and the base substrate, i.e., a height of the signal line is greater than a height of the signal line.