An ultrasound diagnostic apparatus according to an embodiment includes processing circuitry. The processing circuitry sets a ROI in ultrasound image data that corresponds to at least one temporal phase and is among moving image data of two-/three-dimensional ultrasound image data acquired while using a region containing a tissue in motion as an image taking target. The processing circuitry obtains first position information of an estimated ROI based on movement information and second position information of an estimated ROI based on information other than the movement information, in ultrasound image data corresponding to the other remaining temporal phases within an acquisition period of the moving image data. The processing circuitry tracks the ROI, by obtaining position information combining the first and second position information based on an index related to reliability of the movement information, as position information of the ROI.